Trainer Soyou met with the girls to help give them fitness tips. The trainees were paired to perform live in groups based on positions they want to debut in: vocal/rap or dance. There are five songs for vocals and rap (Girls' Generation's "Into The New World", BTS' "The Truth Untold", Heize's "Don't Know You", Blackpink's "Ddu-du Ddu-du" and Wanna One's "Energetic") and four songs for dance (Fitz & The Tantrum's "HandClap", Ariana Grande's "Side to Side", Demi Lovato's "Sorry Not Sorry" and Little Mix's "Touch"). Each song had a certain member limit. Starting from the 13th trainee, they began to pick their choices. The top 12 trainees were the last to pick and could choose groups that were full, and chose a trainee to boot from the group. The remaining trainees would be given extra songs (Jax Jones' "Instruction" for dance and BoA's "Merry-Chri" for vocal/rap). Soyou announced that only 30 trainees would remain in the next round and the winner from each category would receive 100,000 votes. Bora showed up as a special MC for the position battle as Lee Seunggi was unable to attend. Vocal/rap and dance teams were shown and after each performance, they were ranked in their groups.
- Some of the performance were performed on episode 6, and the rest were on episode 7. This is a complete list of this challenge's performances.
The rankings from public vote were not displayed for this episode.